Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 22;37(14):1896–1910. doi: 10.1038/s41388-017-0069-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License, which permits any non-commercial use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. If you remix, transform, or build upon this article or a part thereof, you must distribute your contributions under the same license as the original. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/.

PMC Copyright notice

Fig. 1 — USP14 inhibition or silencing downregulates AR protein level in breast cancer cells. a Protein lysates were collected from the indicated breast cancer cells. Western blot assay was used to detect the expression of USP14 and AR proteins. GAPDH was used as an internal control. b Protein lysates were collected from the indicated breast cancer cells stably expressing USP14 shRNA or control shRNA. Western blot assay was used to detect the expression of USP14, GFP, and AR proteins. c Protein lysates were collected from the indicated breast cancer cells treated with the indicated concentrations of IU1 for 48 h. Western blot assay was used to detect the expression of USP14 and AR proteins. d MDA-MB-453 cells stably expressing USP14 or control shRNA or treated with IU1 or DMSO, and then treated with cycloheximide (CHX) for various lengths of time. Western blot assay was used to detect AR and USP14 protein level