Table 1.
Antibodies used in this study
| Antibody | Staining pattern | Clone | Antigen retrieval | Dilution | Source | Interobserver variability (kappa) |
|---|---|---|---|---|---|---|
| ER | Nuclear | SP1 | EDTA pH 9 | 1:80 | Immunologic | 0.959 |
| PR | Nuclear | PgR636 | EDTA pH 9 | 1:500 | Dako | 0.961 |
| MLH1 | Nuclear | G168-15 | EDTA pH 9 | 1:40 | BD | 0.894 |
| PMS2 | Nuclear | A16-4 | EDTA pH 9 | 1:100 | BD | 0.800 |
| MSH2 | Nuclear | GB12 | EDTA pH 9 | 1:20 | Calbiochem | 0.848 |
| MSH6 | Nuclear | EPR3945 | EDTA pH 9 | 1:1000 | Abcam | 0.763 |
| PTEN | Nuclear and cytoplasmic | 6H2.1 | EDTA pH 9 | 1:100 | Dako | 0.724 |
| IMP3 | Cytoplasmic | 69.1 | EDTA pH 9 | 1:50 | Dako | 0.912 |
| β-Catenin | Membranous and cytoplasmic | 14/β-catenin | EDTA pH 9 | 1:200 | BD | 0.911 |
| L1CAM | Membranous | 14.10 | EDTA pH 9 | 1:100 | BioLegend | 0.921 |
| Ki-67 | Nuclear | MIB-1 | Citrate pH 6.0 | 1:40 | Dako | 0.998 |
| ARID1A | Nuclear | HPA005456 | Citrate pH 6.0 | 1:100 | Sigma | 0.796 |
| Stathmin | Cytoplasmic | 3351 | Citrate pH 6.0 | 1:50 | Cell Signaling | 0.824 |
| P53 | Nuclear | DO-7 | Citrate pH 6.7 | 1:200 | Immunologic | 0.901 |
| P16 | Nuclear and cytoplasmic | MX007 | HIER pH 8.0 | 1:60 | Immunologic | 0.779 |