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. 2018 Apr 5;475(7):1253–1265. doi: 10.1042/BCJ20170703

Figure 2. Nec-1s, an inhibitor of necroptosis, does not prevent OGD-induced death of HIVCM.

Figure 2.

HIVCM cells were treated with or without 10 μM Nec-1s, an inhibitor of necroptosis, 10 min prior to and during exposure to OGD for 4 h (A and B) or (C) 24 h treatment with TNF-α (100 ng/ml). Control cells were treated with an equivalent volume of DMSO solvent (final concentration of 0.1%). (A) Quantification of the proportion of PI-positive cells (A) or AxV-positive cells (B) after 4 h of OGD. (C) Quantification of the proportion of PI-positive cells after 24 h of exposure to TNF-α. Data represent means ± SEM of n = 3 replicates. **P < 0.004; *P = 0.02; ns, not statistically significant (P > 0.99), analyzed by one-way ANOVA.