Figure 11.

Ex vivo wire myograph quantification of the contraction force exerted ex vivo by the rat mesenteric arteries (A,B) and calculated degree of vasorelaxation (C,D) in the presence of NONOate (0.5 nM to 15 μM) and the wires based on 35N LT and L605 alloys coated with the biocatalytic multilayered polyelectrolyte coatings (denoted as wire + Enz + NONOate). Control experiments include administering the NONOate in the absence of wires (denoted as NONOate), using the wires and multilayered coatings with no incorporated enzyme (denoted wire + NONOate), and using the samples identical to the experimental group and also containing specific inhibitors of the NO-mediated signaling pathways (denoted as wire + Enz + NONOate + ODQ/IbTX). Data are presented as mean ± SEM, n = 5 or greater. Statistics is shown for comparing the effects mediated by the biocatalytic coatings with those mediated by the NONOate (¤), the coatings with no enzyme (#), and the biocatalytic coatings in the presence of inhibitors (*) and calculated via a two-way ANOVA followed by Tukey’s multiple comparison test.