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. 2018 Feb 14;208(4):1357–1372. doi: 10.1534/genetics.117.300656

Figure 2.

Figure 2

TRACE [Transient CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 coupled with Electroporation] can dramatically increase the gene disruption rate. The TRACE system was used to generate ADE2 deletion mutants in both serotype A (H99 and cku80Δ H99) and serotype D (JEC21 and XL280) strains. Electroporation with no Cas9/sgRNA (single-guide RNA) was done in parallel. The deletion construct was maintained at the same concentration. Red colonies indicate mutants with disrupted ADE2.