Table 3. Transformation efficiency of ADE2 deletion construct with 1-kb arms.

Strain	Deletion construct	Cas9	sgRNA	Red colonies	Total colonies	ADE2 disruption frequency (%)
H99	+	+	+	13	82	15.85
H99	+	—	—	3	111	2.70
cku80Δ H99	+	+	+	29	489	5.93
cku80Δ H99	+	—	—	2	548	0.36
XL280	+	+	+	161	355	45.35
XL280	+	—	—	1	258	0.39

sgRNA, Single-guide RNA.