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. 2018 Feb 22;208(4):1421–1441. doi: 10.1534/genetics.118.300787

Figure 1.

Figure 1

UBC-9 and SMO-1 localize to the nucleus in the C. elegans germline. (A) 3XFLAG::UBC-9 localizes to the nuclear periphery throughout the germline in the wild-type (WT) strain. Premeiotic tip (PMT) and mid/late pachytene are shown. DAPI is blue, 3XFLAG::UBC-9 is red. (B) Schematic of 3Xflag insertion in the N-terminus of the endogenous ubc-9 locus. The tag is inserted directly after the start codon. (C) Endogenous SMO-1 localization in WT, ubc-9(tm2610), and smo-1(ok359) strains. Nuclear localization is present in WT, but in ubc-9 mutants staining is diffuse with no nuclear preference. smo-1 mutants have low levels of background staining with no nuclear enrichment. DAPI is blue, SMO-1 is red. (D) smo-1::mCherry lines with a WT sequence (GG), or a nonconjugatable point mutation (GA), were expressed in WT and ubc-9 backgrounds. Nuclear mCherry expression is visible in the WT smo-1(GG) strain, but not when ubc-9 is mutated. smo-1(GA) strains have diffuse cytosolic staining in both WT and ubc-9 mutants. All images are midpachytene stage. (E) Graph of signal intensity peaks. Representative lines used for analysis taken 50% through the nucleus for SUN-1 and 3XFLAG::UBC-9 are shown.