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. 2018 Feb 22;208(4):1421–1441. doi: 10.1534/genetics.118.300787

Figure 4.

Figure 4

ubc-9(tm2610); mre-11(ok179) double mutants have underdeveloped germlines and have slowed larval development and survival. (A) DAPI-stained half worm of ubc-9(tm2610); mre-11(ok179) double mutant. The middle of the worm is oriented to the left (arrow labeled “vulva” points to its location). The germline is outlined in a yellow dashed line. syp-3::gfp was crossed into the double-mutant background and whole worms were fixed. SYP-3::GFP linearization was observed in nuclei toward the end of the germline indicating that pairing occurs in these mutants. (B) Larval lethality of broods laid from balanced hermaphrodites of various genotypes. All genotypes are balanced over nT1(gfp). Between three and seven replicates of each genotype were counted. Pairwise comparisons (Fisher’s exact t-test) are in the supplement. Percentages of GFP+/− larvae and GFP+/− adults were calculated to determine if there is increased lethality among the GFP− (homozygous) offspring. Mean ± SEM is given for all percentages.