Figure 5.

Pathway and gene family analysis of hda108 differentially expressed genes. (A) Log₂ fold change heat map of transcription factor coding genes differentially expressed in mutant leaf. TFs were identified according to MapMan (Thimm et al. 2004; Usadel et al. 2009) and Grassius database (http://grassius.org/) annotations. (B) MapMan functional categories enriched in hda108 up- and down-regulated genes in postmeiotic (PMeA) and mitotic (MiA) anthers. Z-scores automatically calculated from P-values (e.g., 1.96 corresponds to a P-value of 0.05) are plotted in an orange to brown color scale. (C) Log₂ fold change heat map of gene families included in the “RNA processing” and “RNA regulation of transcription” categories that are differentially expressed in hda108 PMeA or MiA. The Morpheus (https://software.broadinstitute.org/morpheus/) “Collapse” tool was used to aggregate DE genes belonging to the same gene family and to produce the heat map. (D) Log₂ fold change heat map of gene annotated as pectin-methylesterases (PME) and differentially expressed in hda108 PMeA or MiA.