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. 2018 Feb 12;208(4):1499–1512. doi: 10.1534/genetics.118.300731

Figure 2.

Figure 2

Genetic interactions of type IV collagen mutants with peroxidasin pxn-2. (A) Novel type IV collagen alleles suppress both pxn-2(tm3464) null and spon-1(ju430ts) to viability. (B) Structure of EMB-9 and LET-2 proteins with location of alleles annotated. Novel type IV collagen alleles: ju1166 causes E480K in LET-2, ju1180 causes P588L in LET-2, and ju1197 causes P141L in EMB-9. (C) Loss of function in pxn-2 synergistically enhances the lethality of weak type IV collagen let-2 or emb-9 mutants. A null mutation in pxn-1 does not show synergistic enhancement with type IV collagen alleles. Additionally spon-1 loss of function is unaltered by type IV collagen loss of function. (D) Time series of emb-9(b189); pxn-2(ju358) embryo from comma to arrest. (E) Transgenes containing type IV collagen DNA can partially rescue lethality of pxn-2(lf), but not spon-1(lf). Results of Student’s t-test are indicated for percent total lethality (sum of embryonic and larval lethality). * P < 0.05, ** P < 0.01, *** P < 0.001.