Table 1. Summary of suppressors.

Gene	Allele	Chromosome	Residue change	Codon change (wild type/mutant)	Mutation identificationa/strain	% Lethality in ju430b	% Lethality in tm3464
—	—	—	—	—	—	99.8	100
vab-10	ju958	I	D941N	Gat/Aat	WGS/mapping CZ13802	79.3	55.1
vab-10	ju1299	I	ΔD929-A937	27 bp Δ	CRISPR	ND	(31.2 in ju358c)
vab-10	ju1300	I	ΔR931-D941	33 bp Δ	CRISPR	ND	(35.1 in ju358c)
unc-52	ju1168	II	C402Y	tGc/tAc	WGS CZ21257	6.0	2.4
unc-52	ju1172	II	R1442C	Cgt/Tgt	WGS CZ23560	51.0	11.4
unc-52	ju1174	II	R175C	Cgc/Tgc	WGS CZ21494	36.5	19.0
unc-52	ju1188	II	E1480K	Gag/Aag	WGS CZ22297	ND	ND
emb-9	ju1197	III	P141L	cCa/cTa	WGS CZ22017	37.2	33.8
let-2	ju1166	X	E480K	Gaa/Aaa	WGS CZ20580	11.0	29.2
let-2	ju1180	X	P588L	cCa/cTa	WGS CZ21496	31.2	23.1
let-805	ju1122	III	E1547K	Gaa/Aaa	WGS CZ18264	38.5	20.6
let-805	ju1123	III	C1424R + R1584K	Tgc/Cgc + aGa/aAa	WGS CZ18265	25.1	6.9
let-805	ju1386	III	C1424R	Tgc/Cgc	CRISPR	24.3	1.7
let-805	ju1165	III	S1594F	tCc/tTc	WGS CZ20579	8.9	4.8
let-805	ju1167	III	G1541E	gGa/gAa	WGS CZ20581	33.7	ND
let-805	ju1170	III	R1076C	Cgt/Tgt	WGS CZ20988	36.8	36.2
let-805	ju1173	III	R970C	Cgt/Tgt	WGS CZ20990	9.3	61.1
let-805	ju1175	III	R1443C	Cgt/Tgt	Resequencing	44.8	ND
let-805	ju1177	III	G1541R	Gga/Aga	Resequencing	32.1	ND
let-805	ju1184	III	G1578E	gGa/gAa	Resequencing	4.5	32.6
let-805	ju1187	III	P1422R	cCa/cGa	WGS CZ22296	29.0	2.5
let-805	ju1190	III	V1052I	Gtc/Atc	Resequencing	47.1	ND
let-805	ju1198	III	A1552V	gCt/gTt	Resequencing	37.4	ND

^aWGS, Whole genome sequencing. Resequencing, Sanger sequencing of let-805 hotspots.

^bat 25°.

^cju358 is a viable, strong loss-of-function allele of pxn-2. The single mutant displays 58.5% lethality.