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. 2018 Apr 6;16:37. doi: 10.1186/s12951-018-0361-2

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Measured DNA hybridization ratios for the AON1 (left) and AON2 (right) sequences, with various mismatch locations. The total fluorescence intensity is measured by integrating the emission spectra, as measured by fluorimeter using a 495 nm excitation. The data is normalized with respect to a sample without mismatches, which represents 1.0 on the y-axis. Error bars represent the standard deviation measured through experimental repetition (N = 4)