Fig. 1.

Experimental timeline summary. Timeline of sample acquisition, synbiotic supplementation, and high-fat diet challenge. Around 18 months of age a small group of juveniles (n = 7) were noted to have loose stools of unknown origin and supplementation with a synbiotic was initiated within a week of symptom onset. Supplementation lasted for 75 days and consisted of psyllium seed and the following probiotic bacteria: Enterococcus faecium, Lactobacillus acidophilus, Lactobacillus plantarum, and Lactobacillus casei. On day 50 (denoted by the asterisk) of the 75-day synbiotic supplementation period the majority juvenile’s stools (n = 5) were observed to return to normal. In addition to synbiotic supplementation, juveniles were HFD challenged at 23 to 24 months of age. Days and months post-supplementation is counted from the time that supplementation ended.