Table 1. Sequences of the oligonucleotides ^a .

Note	Sequences (5′–3′)
Methylated DNA	TTG ACT CTG TGG AGT CCT GCA CGA GAC TAG TCA GTA CAC TGC AAG T
Unmethylated DNA	TTG ACT CTG TGG AGT CCT GCA CGA GAC TAG TCA GTA CAC TGC AAG T
Probe X	TAC TGA CTA GTC TCG
Probe Y	P-TGC AGG ACT CCA CAG AG
Probe X′	CTC TGT GGA GTC CTG CA
Probe Y′	P-CGA GAC TAG TCA GTA
Capture probe	CGA GAC TAG TCA GTA-biotin
Reporter probe	TGA CTC TGT GGA GTC CTG CC
Synthesized reporter probe	TGA CTC TGT GGA GTC CTG CCC GAG ACT AGT CAG TA-biotin

^aIn methylated DNA, the italicized “C” base indicates the C5-methylcytosine (5-mC). In probe Y, the “P” indicates the phosphate group (PO₄) modification at the 5′ end, and the italicized “AG” bases indicate the phosphorothioate (PS) modification at the 3′ end. In probe Y′, the “P” indicates the PO₄ modification at the 5′ end. In capture probe, the 3′ end is modified with a biotin. In reporter probe, the italicized “T” base is modified with a Cy5 molecule. In the synthesized reporter probe, the italicized “T” base is modified with a Cy5 molecule, and the 3′ end is modified with a biotin.