Figure 1.

M81-infected LCLs show evidence for global bidirectional translation of the viral genome. (A) Ribosome profiling of EBV-infected LCLs. The schematic shows representative polysome profiles obtained after sucrose density centrifugation of RNA-protein complexes extracted from LCLs treated with either cycloheximide (CHX) (left) or harringtonine (Harr) (right). The fractions of the gradient collected for library generation are shaded in grey. After collection of the fractions, the RNAs are subjected to nuclease digestion and the ribosome-protected fragments (RPF) are used to generate sequencing libraries. NGS: next generation sequencing. (B) The upper panel shows an overview of M81-specific ribosome-protected transcripts after cycloheximide treatment. Reads that map to rightward oriented transcripts are shown in green, those that map to the leftward transcripts are shown in red. The lower panel shows the main EBV transcripts. (C) Same as in (B) but with cells infected with B95–8 and treated with cycloheximide. Please also see Supplementary Figures S1–S3.