Figure 2.

SAXS analyses of apo-RIG-I and RIG-I:RNA complexes ± ATP analogues. (A) Experimental scattering profiles of DC- and FL-RIG-I, with or without 10 bp or 8 bp and ATP analogues, obtained by plotting the log of scattering intensity [log I(s)] as a function of forward scattering vector in the units of Å⁻¹ and represented by black spheres along with gray error bars showing the mean ± SD, (B) pairwise distribution [P(r)] profiles obtained from the scattering data by plotting P(r) as a function of r in the units of Å, (C) normalized Kratky profiles of DC- and FL-RIG-I apo and with dsRNAs in the absence or presence of any ATP analogues. Note: SAXS data for apo-RIG-I constructs in the presence of AMP-PNP are reproduced from (28) with permission for comparative purposes.