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. 2000 Feb;122(2):369–378. doi: 10.1104/pp.122.2.369

Figure 1.

Figure 1

Expression of different nitrilase genes during clubroot development in Arabidopsis. Control (⋄) and infected (♦) roots of transgenic Arabidopsis plants harboring the uidA gene under the control of NIT1-4 promoters were analyzed at different time points (14–35 dpi) for GUS activity. Labeling of the y-axes (GUS activity) is meant for all panels except RNA gel blot. Values are means ± se from different plants of the same experiment. Total GUS activity was calculated by addition of the activities obtained for NIT1-4::uidA plants. At 32 dpi a RNA gel-blot analysis was conducted with control roots (C), separately harvested root (R), and hypocotyl (H) clubs, as well as roots from infested soil showing no symptoms (NS). Nitrilase was detected with a NIT1-cDNA probe. Equal sample loading was confirmed by hybridizing the same blot with an 18S-rRNA probe. MU, Methylumbelliferone.