Figure 4.

Detection of nitrilase from genomic DNA of Arabidopsis and P. brassicae by DNA gel-blot analysis. Lanes A and B show the digested genomic DNA of Arabidopsis and P. brassicae, respectively, prior to blotting after separation on a 0.8% (w/v) agarose gel. From left to right: BamHI, EcoRI, and HindIII digest. Southern blot from genomic DNA of Arabidopsis (lane C) and P. brassicae (lane D) digested with EcoRI was performed with a NIT1-cDNA probe. No signals are visible in the lane with P. brassicae DNA.