Figure 1:

Neighbour joining tree showing clustering of (A) cathepsin B and (B) tenascin gene families. Genes were retrieved by gene name search on GiardiaDB. Gene sequences were downloaded and aligned using ClustalW generated with the MEGA 6 software package. Maximum composite likelihood method was used, with 2000 bootstrap replicates. Bootstrap values greater than 50% are shown above the branches. ♦Proteins confirmed to be secreted using our proteomic analysis.