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. 2017 May 24;18(7):1123–1138. doi: 10.15252/embr.201643827

Figure 4. BAT‐specific deletion of Mfn2 prevents HFD‐induced insulin resistance.

Figure 4

  1. Glucose tolerance test (GTT) on n = 5–7 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group at 5 months old, fed chow diet.
  2. GTT on n = 10 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group, fed a HFD at 22°C.
  3. GTT on n = 5–7 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group, fed a HFD at thermoneutral temperature (30°C).
  4. Insulin tolerance tests (ITT) on n = 8–10 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group, fed a HFD at 22°C.
  5. ITT on n = 5–7 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group, fed a HFD at thermoneutral temperature (30°C). Glucose injection was required due to hypoglycemia 60 min after insulin injection to some BAT‐Mfn2‐KO mice.
  6. Representative images of H&E staining of the liver sections isolated from wild type (WT) and BAT‐Mfn2‐KO (KO) female mice, fed a HFD at thermoneutrality (30°C).
  7. Quantification of the integrated density of lipid droplet signal in liver sections from n = 5–6 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group fed a HFD at thermoneutrality. Each dot represents a mouse and values shown are expressed as arbitrary units. Values shown are average ± SEM. Student's t‐test, unpaired P > 0.05.
  8. Quantification of the integrated density of lipid droplet signal in liver sections from n = 4–8 wild type (WT) and BAT‐Mfn2‐KO (KO) female mice per group fed a HFD at 22°C. Each dot represents a mouse and values shown are expressed as arbitrary units. Values shown are average ± SEM. Student's t‐test, unpaired P > 0.05.
Data information: Values in panels (A–E) are average ± SEM. * represents significance using two‐way ANOVA test, P < 0.05.