Figure 2. HDAC3 is important for growth factor‐induced AKT deacetylation and polyubiquitination.

• A
  293T cells were transfected with plasmids for Flag‐HDAC3 and Myc‐AKT followed by IP and Western blots with the indicated antibodies.
• B
  C4‐2 cells were transfected with plasmids for Myc‐AKT, HA‐Ub, and Flag‐HDAC3 (different doses) for 24 h followed by IP and Western blots with the indicated antibodies.
• C
  C4‐2 cells were transfected with plasmids for Myc‐AKT and Flag‐HDAC3 (different doses) for 24 h followed by Western blots with the indicated antibodies.
• D, E
  C4‐2 cells were transfected with a pool of control or HDAC3‐specific siRNAs for 48 h followed by IP and/or Western blots with the indicated antibodies.
• F
  C4‐2 cells were transfected with HA‐Ub plasmids and treated with 3 μM of RGFP966 for 24 h followed by IP and/or Western blots with the indicated antibodies.
• G
  C4‐2 cells were treated with 10 ng/ml of IGF‐1 for different periods of time and harvested for IP and Western blots with the indicated antibodies.
• H, I
  C4‐2 cells were transfected with a pool of control and HDAC3‐specific siRNAs for 48 h and then treated with IGF‐1 (H) or EGF (I) followed by Western blots for indicated proteins.

Data information: The asterisk (*) indicates the specific HDAC3 protein band. Source data are available online for this figure.