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. 2018 Mar 9;10(4):e8478. doi: 10.15252/emmm.201708478

Figure 4. HDAC3 associates with AKT in plasma membrane and induces lysine 63‐linked ubiquitination of AKT .

Figure 4

  • A
    LNCaP and C4‐2 cells grown in logarithmic phase were fixed and subjected to IFC. Cell nuclei were counterstained by DAPI. Arrows point to the co‐localization of AKT and HDAC3 proteins in the plasma membrane. Approximately 80% of LNCaP cells and 85% of C4‐2 cells showed the co‐localization of these two proteins on plasma membrane. Scale bars, 20 μm.
  • B
    C4‐2 cells were treated with IGF‐1 for 30 min and then harvested for cellular fractionation followed by IP and Western blots with the indicated antibodies.
  • C
    C4‐2 cells were transfected with empty vector (EV) or HA‐tagged wild‐type or mutant HDAC3 for 24 h followed by treatment of 10 ng/ml IGF‐1 for 30 min. Cells were harvested for Western blots with the indicated antibodies.
  • D–H
    C4‐2 cells were transfected with the indicated plasmids for 24 h and harvested for IP and Western blots with the indicated antibodies.

Source data are available online for this figure.