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. 2018 Mar 5;93(6):963–976. doi: 10.1111/tpj.13841

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© 2018 The Authors The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Expression of Arabidopsis cellular markers for embryogenesis (ACE) driven by pRPS5A (ACE‐R) and by pWOX2 (ACE‐W) during embryogenesis

(a) Maximum intensity projection of actin filaments labeled by ACE‐R14 (Lifeact:tdTomato) in the 16‐cell embryo. (b)–(h) Maximum intensity projections of actin filaments labeled by ACE‐W14 (Lifeact:tdTomato) in 1‐cell (b), 2‐cell (c), 4‐cell (d), 8‐cell (e), 16‐cell (f), early‐globular (g), and late‐globular (h) embryos. (i) Overview of ACE‐W14 (Lifeact:tdTomato) expression in the seed. Different acquisition settings were used to accommodate high expression in chalaza. Inset: maximum intensity projection of the 2‐cell embryo in the main panel marked by a dashed box with the acquisition setting used for embryos. Scale bar for (a)–(h) = 5 μm. [Colour figure can be viewed at http://wileyonlinelibrary.com]