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. 2018 Mar 5;93(6):963–976. doi: 10.1111/tpj.13841

Figure 2.

Figure 2

Effects of mounting media on cytoskeletal integrity in embryos

(a), (b) Maximum intensity projections of microtubule (a; ACE‐W15; mGFP:AtTUA6) and actin (b; ACE‐W14; Lifeact:tdTomato) markers in 8‐cell embryos in 5% glycerol and 4% paraformaldehyde in 1 ×  PBS solution. (c), (d) Depth color coding key of all panels illustrated by rotating embryos in (a) and (b), respectively, by 90°. The look‐up table shows color values corresponding to the depth of the image in the z‐dimension. (e)–(l) Maximum projections of microtubule (e–h; ACE‐W15; mGFP:AtTUA6) and actin (i–l; ACE‐W14; Lifeact:tdTomato) markers in 8‐cell embryos imaged in embryo general mounting solution (EGM) (e, i), embryo microtubule mounting solution (EMTM) (f, j), embryo microtubule counterstaining solution (EMTC) (g), embryo general counterstaining solution (EGC) (k) or EMTM followed by the addition of EMTC (h, l).

All images from the same markers were acquired with the same acquisition settings. Scale bar = 5 μm. [Colour figure can be viewed at http://wileyonlinelibrary.com]