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. 2018 Mar 5;93(6):963–976. doi: 10.1111/tpj.13841

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© 2018 The Authors The Plant Journal published by John Wiley & Sons Ltd and Society for Experimental Biology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Endomembrane morphologies in early embryos.

(a), (b) Single optical sections (top rows) and depth‐coded maximum intensity projections (bottom rows) of endosomes and the trans‐Golgi network (a; ACE‐W07; eYFP:VTI12) and Golgi complex (b; ACE‐W09; eYFP:GOT1p) in 4‐cell (left row), 8‐cell (middle row) and 16‐cell (right row) embryos. (c)–(h) Maximum intensity projections of depth‐coded stacks of vacuoles labeled by ACE‐W10 (eYFP:VAMP711) in 4‐cell (c, e, g; left rows), 8‐cell (c, e, g; middle rows) and 16‐cell (c, e, g; right rows) embryos. Panels (d), (f), (h) represent zoomed in images of single cells representing corresponding vacuole morphologies in (c), (e) and (g), respectively, and are shown as single optical sections (left row) or depth‐coded maximum intensity projections (right row). Distinct vacuole morphologies are Type 1 (c, d), Type 2 (e, f) and Type 3 (g, h). (i)–(k) Maximum intensity projection of depth‐coded stacks of nuclear envelopes labeled by the nuclear pore complex marker ACE‐W11 (AtNUP54:GFP) in 4‐cell (left row in i, j), 8‐cell (middle row in i, j) and 16‐cell (right row in i, j) embryos. Panel (i) represents embryos with smooth nuclear envelopes and panel (j) shows embryos with wavy nuclear envelopes. (k) Maximum projection of a depth‐coded stack of an 8‐cell embryo with both smooth (arrows) and wavy (thick arrow) nuclear envelopes, and nuclear pore complexes accumulating in the spindle during mitosis (arrowhead). All images of the same marker were acquired with identical acquisition settings. Scale bars = 5 μm. [Colour figure can be viewed at http://wileyonlinelibrary.com]