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. 2018 Jan 2;218(1):232–241. doi: 10.1111/nph.14957

Figure 5.

Figure 5

Bicarbonate‐activated S‐type anion currents were suppressed in BIG mutant guard cell protoplasts. (a) Typical recording in wild‐type (WT) guard cell protoplasts without bicarbonate. (b) Typical recording of 11.5 mM [HCO 3 −]i‐activated S‐type anion currents in WT guard cell protoplasts. (c) Average current–voltage relationships of whole‐cell currents as recording in (a) (open circles, n = 5) and (b) (closed circles, n = 7). Error bars represent ± SE. (d) Representative recording in doc1‐1 mutant guard cell protoplasts without bicarbonate added to the pipette solution. (e) Representative whole‐cell current recording in doc1‐1 mutant guard cell protoplasts with 11.5 mM [HCO 3 −]i added to the pipette solution. (f) Average current–voltage relationships of whole‐cell currents as recording in (d) (open circles, n = 5) and (e) (closed circles, n = 8). Error bars represent ± SE. (g) Representative recording in big‐1 mutant guard cell protoplasts without bicarbonate added to the pipette solution. (h) Representative whole‐cell current recording in big‐1 mutant guard cell protoplasts with 11.5 mM [HCO 3 −]i bicarbonate added to the pipette solution. (i) Average current–voltage relationships of whole‐cell currents as recording in (g) (open circles, n = 6) and (h) (closed circles, n = 8). Error bars represent ± SE.