Figure 1.

mre11 mutations suppress the sensitivity of sae2Δ cells to CPT and/or phleomycin. (A, B) Exponentially growing cultures were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT or phleomycin at the indicated concentrations. (C) Position of the amino acid changes in the Mre11 sequence. (D) Protein extracts prepared from exponentially growing cells were analyzed by western blot using anti-HA antibody. The filter was also hybridized with anti-Pgk1 antibodies as loading control.