Figure 4.

Suppression of sae2Δ DNA damage sensitivity by Mre11-H98Y, Mre11-K292E and Mre11-R389C depends on Sgs1-Dna2. (A, C, D) Exponentially growing cultures were serially diluted (1:10) and each dilution was spotted out onto YEPD plates with or without CPT or phleomycin at the indicated concentrations. (B) Sporulation efficiency of diploid strains homozygous for the indicated mutations. Data are expressed as percentage of sporulation relative to wild type that was set up at 100%. Plotted values are the mean values with error bars denoting S.D. (n = 3). (E) Meiotic tetrads were dissected on YEPD plates that were incubated at 25°C, followed by spore genotyping.