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. 2018 Feb 6;46(6):2990–3008. doi: 10.1093/nar/gky086

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Effects of H98Y, K292E and R389C substitutions on Sgs1, Rad9, Tel1 and Mre11 association to DSBs. (A–D) ChIP analysis. Exponentially growing YEPR cell cultures were transferred to YEPRG at time zero. Relative fold enrichment of the indicated fusion proteins at the indicated distances from the HO cleavage site was determined after ChIP with anti-HA antibody and subsequent qPCR analysis. Plotted values are the mean values with error bars denoting S.D. (n = 3). *P< 0.05 (Student's t-test).