Figure 1.

Identification of 17 glucose-sensitive phosphoproteins from 32 mRNA-binding proteins. (A) Scheme of phosphoprotein screening. C-terminally TAP-tagged strains for 32 mRNA-binding proteins were subjected to glucose deprivation for 30 min and analyzed by standard SDS-PAGE and Phos-tag SDS-PAGE. Proteins with changed or unchanged phosphorylation status are indicated in the pie chart. (B) Thirteen proteins that were hyperphosphorylated under glucose deprivation conditions. AP treatments were for 30 min at 37°C. (C) Four proteins that were hypophosphorylated under glucose deprivation conditions. The results from Phos-tag SDS-PAGE and standard SDS-PAGE are indicated beside the blots.