Figure 2.

The PKA pathway, Snf1/AMPK pathway and TORC1 pathway regulate phosphorylation of mRNA-binding proteins under glucose deprivation conditions. (A and B) BCY1 or SNF1 was deleted from TAP-tagged strains for 17 glucose-sensitive phosphoproteins, and their phosphorylation status was compared with that of WT cells under glucose deprivation conditions. The results from Phos-tag SDS-PAGE and standard SDS-PAGE are indicated beside the blots. (A) PKA-dependent phosphoproteins found in this study. Dephosphorylation of Eap1 in bcy1Δ strain was detected only in standard SDS-PAGE. Arrowhead, phosphorylated Eap1. (B) Snf1/AMPK-dependent phosphoproteins found in this study. (C) TORC1-dependent phosphoproteins found in this study. Cells were treated with 200 ng/ml of rapamycin for 30 min and subjected to western blotting. (D) Cycloheximide-sensitive phosphoproteins found in this study. Cells were treated with 100 μg/ml cycloheximide for 30 min and subjected to western blotting.