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Impact of blocking shuttling protein recycling on pre-rRNA composition of nucleolar and nucleoplasmic pre-60S particles. RNA was extracted from an aliquot of the purifications shown in this figure, separated by agarose gel electrophoresis and analyzed by northern blotting using radiolabeled oligonucleotides directed to the indicated precursor rRNAs. For comparison, northern blot analysis of total RNA from the crude extract (CE) is also shown.
