Figure 4.

Control of activity and energy expenditure by leptin action on LepRb^Calcr neurons. The 9- to 11-week-old male Calcr^eGFP-L10a mice (Controls; n = 12) and LepRb^CalcrKO mice (n = 8) were subjected to Comprehensive Laboratory Animal Monitoring System (Columbus Instruments) analysis to determine (a, b) locomotor activity; (c, d) Vo₂ normalized to total body mass; and (e, f) Vo₂ adjusted to lean body mass. Data are shown for (a, c, e) dark cycle (Dark), light cycle (Light), and 24 hours (Total) and (b, d, f) the entire hour-by-hour 72-hour period. (g) Graph shows 24-hour Vo₂ consumption rate plotted against body weight for each animal. (b, d, f, g) Mean ± standard error of the mean is shown. **P < 0.01; ****P < 0.0001.