Figure 1.

NR4A1 overexpression and effects of DIM-C-pPhOH and metformin. C2C12 cells were transfected with NR4A1 expression plasmid, and effects on (A) glycolytic gene mRNA levels and (B) NR4A1 protein expression were determined by real-time PCR and western blots of whole-cell lysates, respectively. (C) Structure of C-DIM/NR4A1 ligands used in this study. (D) C2C12 cells were treated with DMSO (control) or different concentrations of C-DIMs and 1 mM metformin, and effects on glycolytic gene expression were determined by real-time PCR. Met, metformin.