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. 2018 Mar 14;159(5):1950–1963. doi: 10.1210/en.2017-03049

Figure 5.

Figure 5.

Role of AMPK in activation of GLUT4 and glycolytic genes. C2C12 cells were treated for 12 hours with AICAR alone (500 μM) or in combination with DIM-C-pPhOH-3-Cl-5-OCH3 or metformin, and effects on (A) NR4A1, GLUT4, PHKG1, and PFKM and (B) PYGM mRNA levels were determined by real-time PCR. Results are means ± SE (three replicates), and significant (P < 0.05) induction is indicated (*). (C) Cells were treated with AICAR, DIM-C-pPhOH-3-Cl-5-OCH3, and metformin (as described previously), and after 24 hours, whole cell lysates were analyzed in a western blot. Met, metformin.