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Confocal microscopic analysis of Glut4 induction and effects of NR4A1 knockdown. C2C12 cells were transfected with si-Ctl (nonspecific oligonucleotide) or siNR4A1. After 72 hours, cells were treated with DMSO (control), 10 μM DIM-C-pPhOH-3-Cl-5-OCH3, or 1 mM metformin. Cells were then fixed, immunostained with β-actin, Glut4 antibodies, and DAPI, merged, and analyzed by confocal microscopy.
