Figure 8.

NR4A1 knockdown attenuates the effects of DIM-C-pPhOH-3-Cl-5-OCH₃ and metformin. C2C12 cells were transfected with si-Ctl (nonspecific oligonucleotide; white bar) or siNR4A1. After 72 hours, cells were treated with DIM-C-pPhOH-3-Cl-5-OCH₃ or metformin, and effects on (A) NR4A1/Glut4 and (B) glycolytic gene expression were determined. (C) C2C12 cells were treated as described in (A), and whole cell lysates were analyzed by western blots. (D) Cells were treated as described in (A–C), and effects of siNR4A1 on compound-induced glucose uptake were determined (glucose uptake in control cells was set at 1.0). (A, B, D) Results are expressed as means ± SE for three replicate determinations. Significantly (P < 0.05) decreased activity due to NR4A1 knockdown is indicated (*). Conc, concentration.