Figure 6.

Effect of PAO on the amount of PM-associated 14-3-3 proteins. PM was isolated from radish seedlings or cultured Arabidopsis cells untreated (C), pretreated with 100 μm PAO (PAO), pretreated with 5 μm FC (FC), pretreated with 100 μm PAO followed by a treatment with 5 μm FC (PAO,FC), or with 5 μm FC followed by a treatment with 100 μm PAO (FC,PAO). After SDS-PAGE and western blotting, immunodecoration was performed with an anti-N-terminal H⁺-ATPase polyclonal antibody and with an anti-14-3-3 polyclonal antibody.