Figure 7.

Effect of recombinant GF 14-6 and FC on the PM H⁺-ATPase activity of PM isolated from cultured Arabidopsis cells untreated (hatched bars) or treated with 100 μm PAO (white bars). PMs (1.5 μg of proteins) were incubated with or without 5 μm FC and with or without 2 μg of GF 14-6 in 50 μL of assay medium (pH 7.3) without ATP, PK, and PEP at 30°C for 30 min. The volume was adjusted to 100 μL with assay medium added with pyruvate kinase, PEP, and ATP (0.3 mm final concentration); the reaction was carried out for 60 min at 30°C.