Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 24;46(6):2834–2849. doi: 10.1093/nar/gky034

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 2. — PCNA Interacting (PIP) motif of PR-Set7 is not necessary for PR-Set7 degradation and is dispensable for Drosophila development. (A) Immunoblot analysis of whole cell extracts prepared from S2 cells expressing ubiquitously (actin promoter) a wild type (FLAG-PR-Set7, upper panel) or a PIP mutant version of PR-Set7 (FLAG-PR-Set7^PIPmutant, lower panel) treated with cycloheximide (CHX) alone or in combination with the proteasome inhibitor MG132 as indicated. Antibodies and relative amounts of each FLAG tagged PR-Set7 protein normalized to Tubulin are indicated on the left and above each panel, respectively. (B) Optic lobes of third instar larvae of different genotypes, as indicated on the left, were stained with DAPI (left panel) and the mitotic marker anti-H3S10p (right panel) and analyzed by immunofluorescence. W¹¹¹⁸ corresponds to a wild type control line. Act>GAL4 indicates the expression of GAL4 protein by the actin promoter. pr-set7²⁰ corresponds to a loss of function allele of pr-set7. UAS-GFP-PR-Set7 and UAS-GFP-PR-Set7^PIPmutant corresponds to transgenic constructs under the control of an UAS sequence. Scale bar represents 50μm.