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. 2018 Jan 24;46(6):2834–2849. doi: 10.1093/nar/gky034

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© The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Degradation of PR-Set7 at G1/S transition induced by the SCF^Slimb complex contributes to S phase entry and chromatin relaxation. (A–C) Left panels: Representative images of nuclei stained with DAPI in different S2 cells expressing different GFP-tagged PR-Set7 versions (as indicated on top) treated with different RNAi combinations as indicated. Scale bars represent 1 μm. Middle panels: Charts representing the percentage of nuclei with compact chromosomes in different S2 cells lines treated with Slimb/LacZ or Slimb/PR-Set7 RNAi as indicated. The number of nuclei analyzed per RNAi condition was approximately 200. Bars represent standard deviation of the mean calculated from three independent experiments. Star in (A) corresponds to P-value < 0.05 (Student's t-test). Right panels: Representative example of FACS analysis showing cell cycle profiles upon BrdU incorporation in the different S2 cells treated with different combinations upon different RNAi conditions as indicated, n = 3. Percentages of cells in the different phases of the cell cycle are indicated for each FACS profile.