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. 2018 Jan 4;46(6):3152–3168. doi: 10.1093/nar/gkx1304

Figure 1.

Figure 1.

Zinc finger domain architecture and protein expression levels of synthetic MBNL proteins. (A) Schematic of synthetic MBNL proteins that shows organization of zinc finger domains (ZF1–2 (Domain A) and ZF3–4 (Domain B)) and location of HA tag and nuclear localization signal (NLS). The length of the individual segments are proportional to the size of each region of the protein. (B) Representative immunoblot comparing relative protein levels of synthetic MBNL proteins in transfected HeLa cells. (C) Quantification of synthetic protein levels in HeLa cells via western blot against the HA tag (n = 4). Relative levels of each protein were normalized to GAPDH. MBNL-AB expression values were then set equal to 1 and MBNL-AA and MBNL-BB protein levels normalized (data represented as mean ± standard error; **P < 0.01, ***P < 0.001, Student's t-test).