Figure 2.

Synthetic MBNL proteins regulate splicing of minigenes in HeLa cells with different activities. (A–F) Jitter plot representations of cell-based splicing assays using INSR, ATP2A1, Vldlr, TNNT2, MBNL1, and Nfix minigenes, respectively. HeLa cells were transfected with empty vector (mock) or MBNL protein expression plasmids and a single minigene reporter. Percent spliced in (PSI, ψ) (i.e. percent exon inclusion) for each protein treatment was then quantified. Each point is from a single experiment and the line represents the average of all experiments for that condition (at least n = 5 for each protein treatment). Average ψ (± standard deviation) and percent splicing activity (displayed in white) are listed below the representative splicing gels. (^oP < 0.05 versus mock, ^#P < 0.0001 versus mock, ^•P < 0.05 versus MBNL-AB, ^†P < 0.0001 versus MBNL-AB, Student's t-test).