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. 2017 Oct 23;32(2):945–956. doi: 10.1096/fj.201700410R

Figure 4.

Figure 4.

p38 phosphorylation in response to LPS was enhanced by eNOS knockdown. A) Representative Western blot images of HMVECs exposed to siRNA (siControl or sieNOS) for 24 h and then treated with LPS (100 ng/ml) for 1 h (left). Images show band intensity for p-p38, p38, p-JNK, JNK, p-ERK1/2, ERK1/2, or α-tubulin with ratios of phosphorylated protein to respective total protein (right) B) Representative Western blot images of HMVECs exposed to siRNA (siControl or sieNOS) for 72 h (left) with 1 h of LPS and respective ratios of phosphorylated protein to associated total protein (right) (n = 6/group). *P < 0.05 between compared groups, †P < 0.05 between group compared to siControl.