Lorazepam- and sulazepam-induced VASP and ERK1/2 phosphorylation in human ASM cells. A–C) Representative electropherograms for p-VASP, p-ERK, and β-actin generated by capillary electrophoresis using ProteinSimple Wes. D, E) Data represent means + sem values from 8 experiments. For p-VASP, a statistically significant (P < 0.05, ANOVA) treatment effect for lorazepam and sulazepam was observed. For p-ERK, a statistically significant (*P < 0.05, ANOVA) treatment effect for lorazepam was observed.