Figure 7.
SHP2 deficiency sensitizes BMM to apoptosis evoked by AKT inhibitor A6730. Randomly growing BMMs from SHP2mΦCTR and SHP2mϕKO mice were treated with DMSO or the AKT inhibitor A6730 (10 µM). BrdU (10 µM) was added to the culture medium for 1 h, and cells were stained with anti-BrdU antibodies and 7-aminoactinomycin D (7-AAD) and subjected to flow cytometric cell-cycle analysis. Regions 1, 2, 3, and 4 (R1, 2 3, and 4) represent sub-G1, G0/G1, S phase, and G2/M cell populations, respectively. Note that, compared with SHP2mϕCTRs, SHP2mϕKO BMMs are hypersensitive to AKT inhibition. Data shown are representative of the 3 independent experiments.