Table 1.

Primer sequences used for CD46 screening

	Forward primer	Reverse primer	Annealing temperature (°C)
Exon 1	5′- CTGTCCTGCAGCACTGGATG-3′	5′- CACGGCCTGCTGTGAGC-3′	62
Exon 2	5′- AGGGCCTTTCTGTTTTTTCTG-3′	5′- GTAGTGGAATATGTACCCCAA-3′	54
Exon 3	5′- ATTCCCACCCATTCAAAAGAG-3′	5′- GCCTATCTCCATAAAACATCC-3′	54
Exon 4	5′- CCACCCCCTCAAACTACTGTAGTG-3′	5′- AGAAACCTCTTTGGGATCTTTGTTA-3′	62
Exon 5	5′-CATTTCCTTTCCTCTTTTTC-3′	5′-ACACCTGCTTTGTTTATCTGTAGA-3′	52
Exon 6	5′- GTCTCTGTTCACACTGGAAAT-3′	5′- TACATAACGTGCTAAGAACCC-3′	54
Exon 7, 8	5′- CCAAGTGGTTGATCTTCTAAC-3′	5′- ATGGCTATACAAATGTCCTCC-3′	60
Exon 9	5′- ATTGATAAGGCCCTGGTGAAT-3′	5′- CACACATACCCTAGAGCTTAA-3′	60
Exon 10	5′- CCCTATGAGTTTAAAGGATTTTAAGCTT-3′	5′- CCTATGTTTGGGCACCTCATAA-3′	58
Exon 11	5′- GGAGATCCATGTGTTCAACATCTT-3′	5′- TCGGTTTAACCAATTTACAAGCTG-3′	58
Exon 12	5′- TTGACCACTGAAATGTAACCAACA-3′	5′- TGAAGCTGCACAAAAGCATGT-3′	60
Exon 13	5′- ATCCCACTTGTTATGCTACTC-3′	5′- TGCCAATATCTCTTTGCTCAG-3′	60
Exon 14	5′-TCATTTTCTGAATAGGCTTCTGGAAT-3′	5′-GCACTCATGAGAGTGAAACTA-3′	58