Figure 3.

TelAP1 is a component of the telomere complex in Trypanosoma brucei. (A) Volcano plot showing interaction partners of TelAP1. Co-IP was performed in four independent experiments with WT and ΔTelAP1 cells. Precipitates were analyzed by MS. The x-axis shows the log2 fold change of detected proteins between WT and ΔTelAP1 cells. The y-axis represents the P-value. (B) Western blot analysis of TelAP1 Co-IP confirmed interaction of TelAP1 and TbTRF. Twenty-fold more of the pellet and IP samples were loaded compared to IN and SN samples. About 13% of TbTRF input was co-precipitated with TelAP1. W (whole cell lysate), IN (input), P (pellet), SN (supernatant), IP (immunoprecipitate).