Table 1. Optimized multiple-reaction-monitoring (MRM) parameters for GABA, Glu, NE, ACh, DA, 5-HT, HVA, 5-HIAA, DOPAC and DHBA (IS).
Analytes | Q1 (amu) | Q3 (amu) | DP (V) | CE (eV) | tR (min) |
---|---|---|---|---|---|
GABA | 104.1 | 87.0 | 30 | 15 | 0.83 |
Glu | 148.1 | 84.1 | 40 | 18 | 0.90 |
NE | 170.1 | 152.1 | 30 | 10 | 0.96 |
ACh | 146.2 | 87.1 | 55 | 20 | 1.01 |
DA | 154.0 | 137.1 | 20 | 15 | 1.12 |
5-HT | 177.1 | 160.0 | 45 | 10 | 1.91 |
HVA | 183.1 | 121.2 | 37 | 12 | 3.11 |
5-HIAA | 192.0 | 146.0 | 55 | 20 | 5.12 |
DOPAC | 169.0 | 123.0 | 25 | 12 | 4.21 |
DHBA (IS) | 140.1 | 123.0 | 40 | 13 | 1.51 |