Table 3. Summary of accuracy, precision, recovery and matrix effect of the 9 analytes in mice brain (n=6).
| Analytes | Concentration (ng/mL) | Intra-day RSD (%) | Inter-day RSD (%) | Accuracy (RE %) | Recovery (%, mean±SD) | Matrix effect (%, mean±SD) |
|---|---|---|---|---|---|---|
| GABA | 10 | 5.2 | 5.3 | 5.9 | 89.2±9.3 | 88.9±5.1 |
| 150 | 4.2 | 3.1 | 6.7 | 92.4±3.2 | 90.2±1.4 | |
| 800 | 7.2 | 3.2 | −2.2 | 91.3±5.7 | 92.8±1.0 | |
| Glu | 20 | 8.3 | 4.9 | −8.7 | 85.5±3.2 | 89.2±3.1 |
| 300 | 5.4 | 4.7 | −3.1 | 94.2±1.3 | 92.6±6.4 | |
| 1600 | 6.3 | 4.9 | 4.4 | 87.5±3.1 | 88.2±4.1 | |
| NE | 20 | 5.2 | 4.9 | −3.1 | 91.2±3.1 | 92.1±4.5 |
| 300 | 4.3 | 6.1 | 2.9 | 89.5±2.5 | 92.8±5.5 | |
| 1600 | 5.1 | 4.3 | −7.2 | 95.2±3.2 | 91.3±2.9 | |
| DA | 5.0 | 5.3 | 6.1 | −6.2 | 90.2±1.0 | 90.9±4.3 |
| 750 | 5.9 | 6.9 | 7.1 | 86.4±2.9 | 97.1±3.2 | |
| 400 | 2.1 | 3.6 | −2.2 | 92.3±4.8 | 89.1±6.5 | |
| 5-HT | 5.0 | 3.5 | 5.4 | −6.2 | 91.2±1.9 | 92.3±4.2 |
| 750 | 4.1 | 3.2 | −1.8 | 95.3±3.7 | 94.8±3.0 | |
| 400 | 5.4 | 5.3 | −2.9 | 88.5±4.5 | 92.8±3.5 | |
| 5-HIAA | 5.0 | 5.2 | 7.2 | 5.9 | 87.2±3.1 | 86.2±1.4 |
| 750 | 5.8 | 5.1 | 1.2 | 88.7±8.3 | 91.3±4.5 | |
| 400 | 6.7 | 5.2 | 2.2 | 89.2±1.9 | 86.4±2.9 | |
| ACh | 5.0 | 4.4 | 2.3 | −2.8 | 92.1±2.1 | 92.3±4.8 |
| 750 | 2.2 | 4.7 | 5.7 | 88.3±6.7 | 91.2±1.9 | |
| 400 | 2.9 | 3.4 | −5.9 | 91.2±2.5 | 88.2±5.5 | |
| DOPAC | 5.0 | 4.7 | 2.3 | −2.9 | 87.7±1.3 | 89.1±5.5 |
| 750 | 2.5 | 4.6 | −4.1 | 97.1±3.2 | 93.1±4.2 | |
| 400 | 2.7 | 4.4 | −5.1 | 89.1±6.5 | 91.1±2.5 | |
| HVA | 5.0 | 4.8 | 4.4 | −3.2 | 92.3±4.2 | 89.9±8.2 |
| 750 | 5.1 | 5.4 | −2.1 | 93.4±4.8 | 92.2±5.0 | |
| 400 | 3.4 | 4.1 | −2.8 | 92.8±1.0 | 90.6±2.8 |